The influences of fluorescence, scattering, and flocculation in turbid material were interpreted for the scattered fluorescence intensity and wavelength, it has been studied the molecular properties by the spectroscopy of laser induced fluorescence(LIF). The effects of optical properties in scattering media have been found by the optical parameters(${mu}_s$, ${mu}_a$, ${mu}_t$). Flocculation is an important step in many solid-liquid separation processes and is widely used in Photodynamic therapy. The interactions of several colloid particles can come into play which have major effect on the flocculation and LIF process. We measured scattering and fluorescence spectra of the sample for in vitro as function of concentration from lase source to detector. The value of scattering coefficient ${mu}_s$ is large by means of the increasing particles of scatterer. Therefore, Phorphyrin A is larger than Phorphyrin C in scattering intensity ${mu}_s$, but Phorphyrin A is smaller than Phorphyrin C in penetration depth ${delta}$.