Cooperative communication, which transmit signal via arbitrary number of relays, enhances the overall communication performance by providing virtual Multi-Input Multi-Output (MIMO) gain without imposing multiple antennal limitation in physical system. There are two representative relaying protocols, i.e., Amplify-and-Forward (AF) and Decode-and-Forward (DF), where we analyze the performance of cooperative communication by adopting DF relaying protocol applying partially differential modulation. The performance is based on symbol error rate (SER), and the effect of relay location on the performance is analyzed. We also compare the performance of the proposed scheme with the system which uses differential modulation scheme.