Operation and control system has been installed and tested to use energy storage system(ESS) for frequency regulation at 4MW class of ESS demonstration facility installed at Jeju island, Korea in 2013. Simulation tests were performed by programing language C# for power system of Jeju island to develop control algorithm. Site simulation tests were performed in control system itself without connecting power system and energy storage system. Control algorithm was coded, modified and tested to load to controller and communication system and human-machine interface were developed and tested in the process of simulation tests. After similar results to that of simulation tests by programing language were obtained, power system and energy storage system demonstration facility were connected to control system by communication system. Energy storage system for frequency regulation was tested for actual frequency and simulation frequency. The site tests showed the similar results to that of simulation tests and the control systems is possible to be operated for frequency regulation. Faster response of energy storage system for frequency regulation, less costs and less capacity of energy storage systems which cover for frequency regulation of power plants. It is expected that more studies for time-reduction and time-delay elements can make less capacity of energy storage system cover more role of frequency regulation of power plants.