Purpose: This study was conducted in order to prove related practice factors of handwashing with soap and its practices by students in South Korea. Method: The subjects consisted of 700 students. Data were collected in Sep 2013 and were analyzed using descriptive statistics, chi-square test, and logistic regression analysis. Results: Total number of handwashing times was 7.5 per day. 65.1% of the students used soap and most of the students washed their hands for 20 seconds(31.1%). Self-evaluation, awareness of effect for prevention of infectious disease, and need for handwashing education were positive, but evaluation of toilet environment was negative. Most of the students washed their hands for 21 seconds(%), and methods of handwashing were not appropriate. Level of awareness was over 85% in most items. Practice differed significantly according to locations, pattern of school, self-evaluation, awareness of effect for prevention of infectious disease, need for education handwashing, and toilet environment. Conclusion: These findings indicate that the students learned methods of handwashing for its practice in South Korea, and they were considered as general characteristics of handwashing from students. In addition, results mentioned above will be reflected in development of educational programs for improvement of handwashing efficiency.