Objectives : The purpose of this study was to investigate the relationship between psychosocial stress and depression symptoms in dental hygiene freshman. Methods : A self-reported questionnaire of 45 questions was filled out by 207 dental hygiene students in Gyeongnam from December 9 to 11, 2013. Data were analyzed using SPSS WIN 18.0 program for descriptive statistics, t-test, ANOVA. Results : Religion tended to influence on positive effects on academic stress because those having religion had less academic stress. Economic condition also influenced on physical and mental stress in the students($8.52{pm}5.63$)(p=0.000). Low economic status influenced on academic problems(p=0.022), family problems(p=0.014), rational relationship problems(p=0.002), and interpersonal problems(p=0.007). There was no significant difference between future problems(p=0.125) and self-efficacy(p=0.076). The physical symptoms included digestive disorders($16.73{pm}5.37$), cardiac symptoms($14.61{pm}5.51$), headache($9.46{pm}3.59$), insomnia($8.32{pm}3.67$) (p=0.000). The mental symptoms included negative emotions($15.07{pm}3.94$)(p=0.000). Conclusions : In order to reduce the psychosocial stress symptoms in dental hygiene freshman, it is important to develop the stress debriefing program in the future.