Purpose. The purpose of this study was conducted to provide the hospital infection control and personal protection, protection of the technician based on data by examining the relationship of hepatitis B knowledge of AIDS knowledge and attitudes of dental hygiene students and the purpose of the present study. Methods. The research subjects were 390 dental hygiene students in limited and data were collected through structured questionnaires. The collected data is analyzed by spss version 14.0 a statistical program for the frequency and one-way ANOVA, multiple linear regression analysis. Results. The received training students of AIDS knowledge and attitudes were higher, hepatitis B knowledge scores and attitudes about AIDS was a statistically significant difference (p <0.001), The experience clinical practice students was AIDS knowledge and attitudes were hepatitis B knowledge high score and difference was statistically significant(p <0.05), The knowledge AIDS and hepatitis B, higher score showed the attitudes significant increase was found to be a static relationship between two variables by both statistically (p <0.001). Conclusion. In order to provided with a thorough prevention program for infectious disease, strengthen school health education and clinical training to help achieve a mutually constructive relationship with the patient through proper education about infectious diseases.