In general, the entire air supply of a bio-safety laboratory (BSL) should be exhausted on the outside to ensure bio-safety, and the air conditioning system should always be operated to maintain a difference in the room pressure. As a result, the annual energy consumption of such a building is approximately five or ten times higher than that of an office building of the same magnitude. Thus, this study applies an actual operating system that targets BSL. The energy consumption is analyzed using the Energy Plus V8.0 program (an energy analysis program), and five kinds of cases that depend on the energy consumption of the basic BSL system are also analyzed. As a result, the energy consumption in Case 1 (basic system) is of 324.95 GJ. When the basic system of Case 1 is compared to that in Case 2 (basic system+passive design with exterior envelopes), an annual energy savings of is 6.9% is achieved. For Case 3 (basic system+Photovoltaic, PV) 12.7% is achieved, and for Case 4 (Solar Geothermal Hybrid System of renewable energy, SGHS) 49.5% is achieved. If a passive design with exterior envelopes and renewable energy system (PV+SGHS) is combined, as in Case 5, the energy consumption would be 118.15 GJ. Therefore, when this last system is compared to a basic system, the passive design with exterior envelopes and renewable energy system (PV+SGHS) can reduce energy consumption by 63.6%.