Purpose: The purpose of this study was to investigate muscle activation related to postural stability depending on different head positions with whole body vibration (WBV) in standing. Methods: Eighteen healthy subjects voluntarily participated in this single-group, repeated-measures study in which the surface electromyography (EMG) data from upper trapezius, rectus abdominis, external oblique abdominis, erector spinae, gluteus maximus, rectus femoris, semitendinosus, medial gastrocnemius were collected over 3 different frequencies (0-10-20Hz) and 4 different head positions (neutral, flexion, extension, chin tuck) for each subject on WBV while standing. Results: The results of this study demonstrated that the EMG activity of all recorded muscles shows significant difference between three different frequencies and four head positions of WBV while standing (p<0.05). In the multiple comparison, significant differences could be observed for most of different frequency conditions except 0-10Hz of RA, 10-20Hz of ST. In contrast, no significant difference showed the comparison of the EMG activity depending on different head positions (p<0.05). Conclusion: These findings suggest that different head positions on WBV do not activate muscles related to postural stability. However, higher frequency on WBV is highly effective to activate whole body muscles included postural muscles regardless of different head positions.