Rearing experiments were conducted using several regimes combined with different photoperiods and water temperatures to investigate the role of temperature and photoperiod as environmental cues regulating reproduction in red marbled rockfish, Sebastiscus tertius. The initial gonadosomatic index (GSI) was $1.62{pm}0.32%$ and that in the $15^{circ}C$ control was $7.01{pm}0.46%$ at the end of the experiment. The highest GSI was $9.10{pm}0.35%$ in the 12L:12D photoperiod-treated fish. The highest GSI at $19^{circ}C$ was $15.03{pm}0.86%$ in the 12L:12D photoperiod treatment. The initial hepatosomatic index (HSI) was $3.09{pm}0.72%$ and that in the $15^{circ}C$ control was $3.88{pm}0.45%$. The highest HSI was $4.00{pm}0.56%$ in the 15L:9D treated fish. The highest HSI at $19^{circ}C$ was $4.05{pm}0.63%$ in the 12L:12D treated fish.