Modern commercial greenhouse requires the use of advanced climate control system to improve crop production and to reduce energy consumption. As an alternative to classical sensor-based control method, this paper introduces a model-based control method that consists of two models: the predictive model and the evaluation model. As a first step, this paper presents straightforward models to predict the effect of natural ventilation in a greenhouse according to meteorological factors, such as outdoor air temperature, soil temperature, solar radiation and mean wind speed, and structural factor, opening rate of roof ventilators. A multiple regression analysis was conducted to develop the predictive models on the basis of data obtained by computational fluid dynamics (CFD) simulations. The output of the models are air temperature drops due to ventilation at 9 sub-volumes in the greenhouse and individual volumetric ventilation rate through 6 roof ventilators, and showed a good agreement with the CFD-computed results. The resulting predictive models have an advantage of ensuring quick and reasonable predictions and thereby can be used as a part of a real-time model-based control system for a naturally ventilated greenhouse to predict the implications of alternative control operation.