Railway operators have given a lot of efforts to determine the railway route of the minimum cost. In order to determine the optimal alignment, the alignment should be allocated satisfying the design criteria on various geographical condition with the minimum earth works. The determination of the optimal railway alignment is a kind of combination optimization because that must consider various design elements. This study developed a numerical model to determine the optimal railway alignment with the minimum construction cost. The problem was analyzed by the genetic algorithm, and the concept of the optimal alignment was established with the results from the analyses. The methodology was applied to a fictitious rail construction section and the result was evaluated. This methodology is meaningful considering the fact that the cost for energy is greater than that of the construction.