This study was conducted to apply microcurrents of $500{mu}A$ effective for ATP generation to leg muscles to which delayed onset muscle soreness (DOMS) had been induced in order to examine whether the microcurrent stimulation was effective for gait improvement. Forty subjects were randomly assigned to an experimental group of 20 subjects and a placebo group of 20 subjects. Microcurrents were applied immediately after inducing DOMS and 24 hours and 48 hours thereafter and changes in the center of pressure (COP), cadence, and affected stance phases were measured. According to the results of the measurement, changes in the COP, cadence, and affected stance phases at 48 hours after the induction of DOMS were statistically significant compared to the values before the induction. The COP of the experimental group became statistically significantly lower and the ratio of affected stance phases of the experimental group became statistically significantly higher compared to the placebo group at 48 hours after the induction of DOMS. Therefore, $500{mu}A$ microcurrents showed effects for gait improvement by promoting the recovery and healing of damaged muscles.