This research measures changes in hair loss and gain, and subjective user experience after applying shampoo that is made of natural organic ingredients. Participants were instructed to apply the shampoo twice a day and rinse five minutes after applying it. The data obtained from the research was analyzed with SPSS ver.21.0. The results are as follows: 1. In the experiment group, some items appeared to improve. The following are such items that improved during this study: refreshed sensation in scalp, less itchy, thickened hair, nutritional supplements unwanted, and softened hair (p<0.001). 2. In the experiment group, some items appeared to degrade. The following items appear to degraded during this study: itchy hair, and smelly hair (p<0.001). As a result of measuring the quantity of hair, the number of hair loss for four participants gradually decreased. 3. After applying the shampoo for four months, the participants’ hair condition improved significantly. In conclusion, shampoo that is made of natural ingredients seems to decrease hair loss and improve of rate of hair gain. Therefore individuals suffering from hair loss may expect some improvement in controlling their hair condition.