The purpose of this study was to examine the influence of teachers' vocational aptitude and sense of responsibility on their teaching efficacy in early childhood education and care. 259 teachers in ECEC were recruited from 19 daycare centers and the data were analyzed through frequency, percentage, Pearson's correlations, and stepwise regression using SPSS 18.0. The findings are as follows. First, teachers’ teaching efficacy was significantly different according to their age, the number of years of teaching experiences, and education levels. Second, all degrees of teachers’ vocational aptitude, sense of responsibility, and their teaching efficacy were higher than the median points. Second, teachers’ efficacy relating with running class was affected most by the ability of safe management in vocational aptitude, and teaching methods in sense of responsibility. For teachers’ efficacy relating to teaching, acceptability/sensitivity in vocational aptitude and motivation had the most impact. The implication was discussed in order to figure out the ways to increase teachers’ efficacy.