Cells throughout the body, especially the intestines, liver, and kidneys, produce blood ammonia. The liver uses most of the ammonia in the body during the production of urea, which is also a waste product, but it is much less toxic than ammonia. Ammonia is especially toxic to the brain as it can cause confusion, lethargy, and sometimes even comas. Blood ammonia measurements are kept in refrigerated temperatures then transported and analyzed within 30 minutes. In this study, the rate of increase of blood ammonia over time was compared between blood samples kept in room temperature storage and those kept in refrigerated temperature storage. The blood samples stored at different temperatures were compared with a blood ammonia quantitative analysis conducted at 10 minutes intervals. At room temperature the samples were analyzed for their blood ammonia level; after 20 minutes of storage there was an 8.8% increase, after 30 minutes of storage there was a 19.4% increase, and after 40 minutes of storage there was a 44.4% increase. Then blood ammonia was stored at refrigerated temperature and analyzed at 10 minutes intervals. After 20 minutes of storage the blood ammonia level saw a 6.0% increase, after 30 minutes of storage there was an 18.9% increase, and after 40 minutes of storage the level increased by 40.4%. When analyzing the blood ammonia ensure that the sample is transported and stored in refrigerated conditions. Also, unless it is an exceptional case, the sample should be analyzed without delay for the most accurate measurements.