The purpose of this study was to survey hygiene behavior of college students and adults and to use the results in education and research for promoting hygiene behaviors. The questionnaire survey was carried out with a convenience sample of 268 college students and adults. As a result, college students showed hygiene behavior is significantly lower than adults. Especially college students showed general hygiene and household hygiene significantly lower than adults. However college students showed experience of hygiene education and the ratio of the more than 21 second hand-washing-time for college students is significantly higher than that for adults. Therefore programs and education adapted to the characteristics of each age category are needed to promote hygiene behaviors such as general hygiene, hand hygiene methods, household hygiene, food-related hygiene, and personal hygiene.