Smoking stimulating factors, health behavior and health status of College students are different according to gender. Although there have been many studies on smoking stimulating factors, health behaviors and health status of smoking college students, there are few studies on the difference according to gender among domestic smoking students. The purpose of this study was to investigate the differences in smoking stimulating factors, health behavior and health status according to gender. A questionnaire survey was conducted on 144 College students who were enrolled in a College in U City, Korea from April to June 2015, and their Smoking stimulating factors, health behavior, and health status were measured. The collected data were analyzed using IBM SPSS Statistics 21.0. Independent t-test was used to identify differences in factors stimulating cigarette smoking, health behaviors and health status according to gender. There were significant differences in the number of smokers in the family living in the male and female college students. There was no significant difference in the health behaviors. There were differences in the daily life stress, depression, and subjective health status among the health status. The number of smokers living in a family with two or more family members was higher in males than females(χ2= 10.897, p=.004). On the other hand, there are more female students who feel stress in daily life (χ2= 18.641, p<.001). When respondents answered yes to the feeling of depression(χ2= 17.114, p<.001), when they answered that they were very poor in their subjective health status(χ2= 8.466, p=0.016), they also had more female students than boys. Based on these results, it is necessary to consider Smoking stimulating factors, health behavior and health status according to gender in establishing health care strategies for college students.