Purpose: The purpose of this study was to investigate the detection rate of putative periodontopathogens, Porphyromonas.
gingivalitis, Prevotella. intermedius, Tannerella. forsythensis, Treponema. denticola, Actinobacillus. actionmycetemcomitans,
related to implant-abutment interface microleakage.
Materials and Methods: Samples were taken from 27 subjects with sterilized paper points and were transported in
1*PBS. The detection of periodontopathogens were performed by polymerase chain reaction with species-specific primers
based on 16S rDNA. The PCR products were cloned into pGEM-T easy vector and its nucleotides were sequenced
in order to confirm the specificity.
Results: Our data showed that the detection rate of Porphyromonas. gingivalitis and Prevotella. intermedius in implant
fixture was 59% and 82% in patients respectively. Detection rate of Porphyromonas. gingivalitis and Prevotella. intermedius
in implant crevice was 44% and 82% in patients. Detection rate of Porphyromonas. gingivalitis and Prevotella.
intermedius in tongue was 82% and 82% in patients.
Conclusion: Current implant systems cannot safely prevent microbial leakage and bacterial colonization of the inner
part of the implant. (JOURNAL OF THE KOREAN ACADEMY OF IMPLANT DENTISTRY 2011;30(2):104-114)