최근 자가골을 대체하기 위한 이식재로서, 생체 유래 재료에 대한 안전성에 대한 관심이 높아지며 자가 혈액이나 자가 세포 및 조직을 이용한 연구가 활발히 진행되고 있다. 이런 노력들 중 탈회상아기질 (Demineralized Dentin Matrix)은 Urist에 의해서 처음으로 동 물실험이 이루어졌으며1) 골유도 효과가 있음이 보고되었다. 탈회상 아기질을 이용한 다른 동물 실험들에서도 신생골 형성이 되었다는 보고되었으며2-6) 최근 임상에 활용되기 시작하였다7,8). 하지만, 앞서 언급한 논문들에서는 0.6 N 염산을 이용한 탈회시간이 장시간 요구 되고 있어9,10) 임상 활용시 발치한 치아를 즉시 탈회상아기질로 제작 후 즉시 이식하기에는 제약이 있었다. 그러나 2% 질산을 이용해 20 분간 탈회하는11,12) 방법이 소개됨으로써 임상에서의 활용이 유용할 것으로 사료된다.
Purpose: Some studies have reported that Autogenous demineralized dentin matrix (ADDM) granules which were demineralized with 2% HNO₃ for 20 minutes have osteoinduction and osteoconduction. The aim of this study is finding out the proper demineralization time for using ADDM block as the graft material in comparison previous studying that evaluate the proper demineralization time ADDM granules which were demineralized with 2% HNO₃. Materials and Methods: The enamel of crown was removed and the dentin was shaped as a rectangular parallelepiped for making tooth block (8×8×5 mm). In tooth-blocks, 1 mm diameter holes were formed every 1 mm intervals. Each tooth-blocks were demineralized in 2% HNO₃ solution for 1, 2, 3 hours. The change of weight, radiological density and minerals (Ca, P) were measured. Results: This result has showed that ADDM block which was demineralized by 2% HNO₃ is needed to have 2 hours demineralization time. But when applying this to clinical cases, we should consider that it is needed to take subdivided times for the better efficient, economical and stable demineralization time and histological studies through additional animal test. Conclusion: These results of ADDM blocks were compared with the results of ADDM granules that were already confirmed to evaluate proper demineralization time for using the ADDM block as graft material. (JOURNAL OF DENTAL IMPLANT RESEARCH 2012;31(2):44-48)
서 론 대상 및 방법 결과 고찰 결론 REFERENCES
