Purpose: The purpose of this study was to examine the effect of laughter therapy on arthralgia, ankylosis, depression and sleep of elderly housebound women suffering from osteoarthritis. Methods: The study used a nonequivalent control group pretest-posttest design. The participants were 48 elderly women: 23 in the experimental group and 25 in the control group. The experimental group received laughter therapy twice a week for 4 weeks. The results were analyzed by using X2-test, ANCOVA, and t-test with the SPSS/WIN 21.0 program. Results: The results showed that laughter therapy had a significant statistical effective in reducing arthralgia, ankylosis, and depression and improving the quality of sleep as evidenced by the differences between the two groups. Conclusion: The results of this study indicated that laughter therapy is an effective nursing intervention reducing arthralgia, ankylosis, and depression and improving the quality of sleep in these women. Therefore, it is necessary to develop laughter therapy as an independent nursing intervention for elderly women with osteoarthritis who are housebound.