In order to evaluate the accuracy of the measurement of maximal oxygen intake (MOI), the MOI in seven subjects was determined 3 to 4 times in each individual. Following a 10 minute warm-up on treadmill (4.3 km/hr with 9 degree grade), the subject was asked to run at a speed of 8.73 km/hr on treadmill for a period of 3 minutes at a given grade which was elevated in a step-wise manner from zero to the level of exhaustion. Following a 3 minute run, the subject was allowed to rest for a period of 3 to 5 minutes. During each period of running, several cardio-pulmonary functions were determined and the range of variability for each measurement was computed. The oxygen consumption during the maximal work load was taken as the MOI. The results may be summarized as follows: (1) The minute volume, the oxygen consumption and the heart rate increased linearly until the grade was elevated to 9 degree above which these values were leveled off. (2) The minute volume and the heart rate during maximal exorcise were 87.4±8.10 l/min and 187±3.7 per minute, respectively. (3) The maximal oxygen intake which corresponds to the oxygen consumption during maximal exercise was averaged to 3.04 1/min. (4) The coefficient of variance for the maximal oxygen intake was 6.32% while the corresponding values for the minute volume and the heart rate during maximal exercise were 5.22 and 2. 14%, respectively.