This study was undertaken to examine the effect of ethanol on Na⁢-dependent transport systems (glucose, phosphate, and dicarboxylate) in renal brush-border membrane vesicles (BBMV). Ethanol inhibited Na⁢- dependent uptakes of glucose, phosphate, and succinate in a dose-dependent manner, but not the uptakes of Na⁢-independent. The H⁢/TEA antiport was reduced by 8% ethanol. Kinetic analysis showed that ethanol caused a decrease in Vmax of three transport systems, leaving Km values unchanged. Ethanol decreased phlorizin binding, which was closely correlated with the decrease in Vmax of Na⁢-glucose uptake. These results indicate that ethanol inhibits Na⁢-dependent uptakes of glucose, phosphate, and dicaboxylate and that the reduction in Vmax of Na⁢-glucose uptake is caused by a decrease in the number of active carrier proteins in the membrane.