Objectives: This study is to confirm the effect of combined extract and individual extract of Samjunghwan (SJH) in anti-oxidative and anti-obesity effect. Methods: Combined ethanol extract of readily made SJH and individual ethanol extract of Atractylodes japonica, Cortex lycii radicis, and Morus alba Linne was combined after the extraction. To evaluate the anti-oxidative effect of SJH, total phenol compound and2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging ability were conducted. Real-time quantitative-polymerase chain reaction analysis of transcription factor peroxisomeproliferator-activated receptror γ (PPARγ), adenosine monophosphate-activated protein kinase(AMPK)-α1, tumor necrosis factor-α (TNFα) and 3-hydroxy-3-methylglutaryl CoA reducatase(HMG-CoA reductase) were done with 3T3-L1 cells to investigate the ant-obesity effect. Also,cell viability analysis were done to see to toxicity of SJH.Results: Individual extract of SJH showed significant decrease in TNFα and AMPK transcription while PPARγ showed significant increase. Combined extract and individual extract of SJH both showed decrease in HMG-CoA reductase. DPPH free radical scavenging ability and total phenolcompound was analogous between two groups. Conclusions: Individual extract of SJH appears to be more effective in anti-oxidation andanti-obesity effect compared to combined extract of SJH.