Tooth movement by segment is one of the means which are frequency used in daily orthodontic practice. When we retract or intrude a tooth or teeth, we should recognize the center of resistance of the certain tooth or teeth. There have been many studies about the center of resistance of a single tooth, not so much was about the tooth-segment. At the present study the center of resistance of the maxillary anterior segment is experimentally investigated by using laser reflection technique and metal splints on the human dry skull. The variables of intrusive force magnitude are divided into two groups, 50g and 100g groups. The results were as follows ; 1. The center of resistance of the maxillary anterior segment composed of the central and lateral incisors was at the mesial portion of canine crown at the coronal level. 2. The center of resistance of the maxillary anterior segment composed of the central and lateral incisors and canines is between the canine and the 1st premolar crowns at the coronal level.