The purposes of this study were to investigate the effects of forest or ground walking exercise program on the health-related fitness and functional movements in 50s females. For those purposes, the subjects were divided into forest walking group and the ground walking group, both of which participated in 80 minutes per session and three times per week for 10 weeks. Health-related fitness(Cardiovascular endurance, Muscular strength and endurance of leg, Flexibility, and Fat mass) and functional movements(Deep squat, Huddle step, Inline lunge, Shoulder mobility, Straight leg raise, Push-up, Rotary stability) were measured before and after the 10-week exercise program. As a result, First, the subjects who participated in the forest walking exercise program showed significant improvement in all health-related fitness levels(Cardiovascular endurance, Muscular strength(Extensor and Flexor strength of dominant leg), Flexor endurance of dominant leg, Flexibility, and Fat mass). but those who participated in the ground walking exercise program did not show any significant changes in the factors related to the health-related fitness except muscular endurance of dominant leg and fat mass, Second, the subjects who participated in the forest walking exercise program showed significant improvement in inline lunge and rotary stability in functional movement screen. but those who participated in the ground walking exercise program did not show any significant change in the items measuring functional movements, Therefore, this study suggests that forest walking exercise can be a more effective solution for the improvement of physical fitness and functional movements of 50s females.