Although the incidence rate of cervical cancer has recently tended to be gradually decreased in Korea, the rate in those aged 10-34 ranks No. 3, so cervical cancer becomes a risk factor of females genital health. In particular, the HPV infection which has been known as a main cause for cervical cancer has the highest prevalence among those in their 10s and 20s. This study, therefore, aims to provide basic data of health education intervention programs for female college students, by examining their knowledge of cervical cancer and HPV infection and the relationship between it and their intention for preventive behavior. The subjects include 192 female students in three colleges located in B metropolitan, and data were collected from October, 22 to November, 25, 2013. The collected data were analyzed with the real number and percentage, average, standard deviation, average ratings, t-test, ANOVA and Scheffe s test, by using IBM SPSS 21.0. The findings show: the rate of correct responses to items regarding female college students knowledge of cervical cancer was low(46.8%); the rate of correct responses to items regarding their knowledge of HPV infection was very low (31.1%); and preventive behavior intention for cervical cancer and HPV infection was high (77.5%). Although there was a significant correlation between their knowledge of cervical cancer and HPV infection, knowledge of both them had no significant relationship with preventive behavior intention. On the basis of the findings, the development of health education programs is required for enhancing knowledge of HPV infection, a main cause of cervical cancer. In addition, it will be necessary to consider medical check-ups for increasing the screening rate of cervical cancer for which female college students had weak preventive behavior intention.