Purpose: This study was done to investigate long-term care hospital nurses’ awareness and ethical attitudes toward Do-not-resuscitate (DNR). Methods: Data were collected from 146 nurses who worked in long-term care hospitals for more than six months. Participants worked in G city and J Province, and the data were collected from October to December, 2018. Data analysis was done using descriptive statistics and x2 test with the SPSS/WIN program version 23.0. Results: There were significant differences in participants awareness of DNR according to age, length of time working as a nurse in a long-term care hospital, position, work pattern, received DNR education, decision maker and the existence of guideline for DNR. There were significant differences in participants ethical attitudes toward DNR according to the length of time working as a nurse in a long-term care hospital, position, DNR education, place of education, experiences with DNR as one who calls for DNR, and decision maker for DNR. Conclusion: Findings suggest that there is a need to develop guidelines for DNR, and also DNR related education programs for nurses in long-term care hospital. Furthermore, qualitative research using in-depth interviews for nurses in these hospital is needed.