The purpose of this study is to verify the moderating effects of the supervisory relationship between self-care and burnout among play therapists. The subjects of this study are 153 play therapist who received the supervision at least three times. For this purpose, Self-Care Inventory, Korean Short Version of Supervisory Relationship Questionnaire (K-S-SRQ), and Burnout Inventory were used. The results of this study can be summarized as follows. First, a correlation analysis was performed to verify the relationship between self-care, supervisory relationship, and burnout. As a result, supervisory relationship and the sub-factors of the supervisory relationship and play therapist’s self-care had a negative correlation with burnout. Second, a hierarchical multiple regression analysis was performed to verify the moderating effect of the supervisory relationship between self-care and burnout. As a result, it was found that the total score of the supervisory relationship and its sub-factors had moderating effects on the relationship of play therapist’s self-care and burnout. In conclusion, this study confirmed the moderating effects of the supervisory relationship between self-care and burnout among play therapists. Through this, it is meaningful to verify the supervisory relationship as one of the protection factors for the prevention of burnout of play therapists.