Purpose: This study was performed to identify the current status of organization and education for evidence-based nursing (EBN) among acute care hospitals in Korea. Methods: Using a questionnaire, the data were collected from 60 acute general hospitals selected conveniently from October to November in 2016. Results: The response rate was 83.3% and the mean number of beds for responded hospitals were 977.83. Responded hospitals appeared to perceive the importance of EBN, though approximately 50% of hospitals showed insufficient status for EBN. EBN project implemented in accordance with institutional support. EBN education courses have operated within hospitals and also have attended at outside courses. Conclusion: The current status of EBN organization and education have identified and it is needed to monitor continuously to activate and expand EBN in clinical settings.