The resistance to crown gall in grape rootstocks was evaluated by inoculating cuttings from 27 grape rootstock varieties with Agrobacterium vitis strain Cheonan 493. Tumors were formed in all varieties of grape rootstocks tested in this study. The symptoms were observed in the stems of all plants tested including ``196-17`` and ``41B``. Based on the measurement of tumor weight on the stems of grape rootstocks, ``779P`` was found to be highly susceptible to crown gall. While some varieties such as ``Gloire``, ``140R``, ``101-14M``, ``3309C``, and ``333EM`` were found to be relatively resistance to crown gall. Varieties such as ``99R``, ``1447P``, ``Rupestris du lot``, ``110R``, ``Freedom``, and ``41B`` were also found to be susceptible to crown gall. Other varieties including ``1103P``, ``Teleki 5C``, ``420A``, ``Golia``, and ``5BB`` were found to be moderately susceptible.