The heat-inactivated (at 121 oC for 20 min) conidial suspension of Bipolaris cactivora (HICS) was evaluated for the control of Bipolaris stem rot of cactus caused by B. cactivora. Severe rot symptoms were developed on the cactus stem discs inoculated with B. cactivora from 5 days after inoculation. However, only small brownish spots developed on the stem discs treated with HICS 2 days prior to the pathogen inoculation. HICS also reduced symptom development on cactus stem discs inoculated with other fungal pathogens such as Alternaria alternata, Colletotrichum gloeosporioides, and Fusarium oxysporum, suggesting its disease-inhibitory efficacy may not be pathogen-specific. HICS significantly reduced severities of the stem rot disease on several cactus species including Hylocereus trigonus, Cereus peruvianus, Chamaecereus silvestrii and Gymnocalycium mianovichii, but not on Cereus tetragonus. Extensive wound periderms were formed in the stem tissues of inoculation and/or wounding sites on C. peruvianus treated with HICS alone or prior to the pathogen inoculation, but not on C. tetragonus, indicating the structural modifications may be related to the mechanism of disease suppression by HICS. HICS also reduced the disease development on the grafted cactus (H. trigonus stock and G. mianovichii scion) with the control efficacy nearly equivalent to the application of a commercial fungicide. All of these results suggest HICS can be used as an environmentalfriendly agent for the control of the cactus stem disease