The purpose of this study was to find out how Accumulation of Short Duration exercises influence on expression of apoptosis genes in a mouse skeletal muscle. The subjects, ICR-type mice, were divided into three cohorts, Accumulation of Short Duration exercise group, continuous aerobic exercise group, and control group. Accumulation of Short Duration exercise group performed wheel-running for fifteen minutes respectively in the morning and afternoon three times a week Continuous exercise group performed wheel-running for thirty minutes three times a week Both split and continuous groups completed the wheel-running trials for sixteen weeks. To identify the difference of apoptosis expression between groups, the data colleted from the skeletal muscle were analysed by one way ANOVA Analyses of the data revealed that significant differences of apoptosis concentration in both continuous and Accumulation of Short Duration exercise groups, there was a notable differences were observed in control group.