The purpose of this study is to determine the effect of the first experience of the clinical experience for psychiatric nursing on the urinary 17-ketosteroid (KS) and 17-hydroxycorticosteroid (OHCS) of the nursing students. We analyzed the urine of fifteen students on curriculum who were students of D college in K city, compared with the control. In terms of 17-KS, at the end(4 PM) of the first day (p=0.001) and the last day(p=0.003), there was statistically significant difference between the control and the experimental group. In terms of 17-OHCS, at the end of the first day, there was statistically significant difference between the control and the experimental group (p=0.005), and between at the beginning (8 AM) and at the end during the clinical experience (p=0.035). At the last day, also, there was significant difference on the statistics between at the beginning and at the end in both the control (p=0.018) and the experimental group (p=0.004). In conculusion, 17-KS and 17-OHCS may be used as a indicator of the amount of stress to improve the educational environment for the students.