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Optimization of Polyethylene Glycol-Mediated Transformation of the Pepper Anthracnose Pathogen Colletotrichum scovillei to Develop an Applied Genomics Approach
Jong-Hwan Shin, Joon-Hee Han, Hyun-Hoo Park, Teng Fu, Kyoung Su Kim 한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 6호 4 575-584 (10 pages)
wide range of host plants. Strain C. acutatum KC05, which was previously isolated from an infected pepper in Gangwon Province of South Korea, was reidentified as C. scovillei using combined sequence analyses of multiple genes. As a prerequisite for understanding the pathogenic development of the pepper anthracnose pathogen, we optimized the transformation system of C. scovillei KC05. Protoplast generation from young hyphae of KC05 was optimal in an enzymatic digestion using a combined treatment... -
Analysis of in planta Expressed Orphan Genes in the Rice Blast Fungus Magnaporthe oryzae
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2014, 30권 4호 4 367-374 (8 pages)
on in planta expressed sequence tag data in the rice blast fungus, Magnaporthe oryzae. Expression analysis using qRT-PCR confirmed the expression of four genes (MoSPC1, MoSPC2, MoSPC3 and MoSPC7) during plant infection. However, individual deletion mutants of these four genes did not differ from the wild-type strain for all phenotypes examined, including pathogenicity. The length, GC contents, codon adaptation index and expression during mycelial growth of the four genes suggest that these genes... -
Proteomic Analysis of a Global Regulator GacS Sensor Kinase in the Rhizobacterium, Pseudomonas chlororaphis O6
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2014, 30권 2호 15 220-227 (8 pages)
The GacS/GacA system in the root colonizer Pseudomonas chlororaphis O6 is a key regulator of many traits relevant to the biocontrol function of this bacterium. Proteomic analysis revealed 12 proteins were down-regulated in a gacS mutant of P. chlororaphis O6. These GacS-regulated proteins functioned in combating oxidative stress, cell signaling, biosynthesis of secondary metabolism, and secretion. The extent of regulation was shown by real-time RT-PCR to vary between the genes. Mutants of P.... -
Research Notes : The RpoS Sigma Factor Negatively Regulates Production of IAA and Siderophore in a Biocontrol Rhizobacterium, Pseudomonas chlororaphis O6
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2013, 29권 3호 11 323-329 (7 pages)
The stationary-phase sigma factor, RpoS, influences the expression of factors important in survival of Pseudomonas chlororaphis O6 in the rhizosphere. A partial proteomic profile of a rpoS mutant in P. chlororaphis O6 was conducted to identify proteins under RpoS regulation. Five of 14 differentially regulated proteins had unknown roles. Changes in levels of proteins in P. chlororaphis O6 rpoS mutant were associated with iron metabolism, and protection against oxidative stress. The P.... -
Some Suggestions for Practical Listening Activities in Elementary English Classroom
Lee Hae-kyoung 공주교육대학교 초등교육연구원 교육논총 15 Pages
공주교육대학교 초등교육연구원 교육논총 2018, 55권 1호 10 1-15 (15 pages)
This paper suggests some practical listening activities in Elementary English classroom: ‘write down the number of the one you hear’, ‘listening grid’, ‘listen and find’, ‘listen and work out the order’. These activities are all intended to encourage children in language exchanges and to develop confidence and willingness to have a go. The activities can be used occasionally to supplement a textbook or they can stand on their own as the basis for an independent programme. Most of the... -
현실치료의 선택이론 단어자극이 인터넷중독 청소년의 사건유발전위에 미치는 영향
양은진 한국현실치료상담학회 현실치료연구 17 Pages
한국현실치료상담학회 현실치료연구 2012, 제 1권 1호 4 57-73 (17 pages)
본 연구는 인터넷 중독 청소년에게 선택이론 단어자극과 긍정/ 외부통제 단어자극과 부정의 연합관 계를 통하여 현실치료의 선택이론 단어자극에 대한 생리적인 변화를 사건유발전위로 규명하고자 하 였다. 연구 결과, 내부통제성의 차이는 인터넷 중독 청소년이 비중독 청소년보다 낮았다. 둘째, 선택 이론 단어자극-긍정/외부통제 단어자극-부정의 연합자극이 선택이론 단어자극-부정/ 외부통제 단어자 극-긍정의 비연합자극보다 평균 반응속도가 빨랐다. 셋째, 연합자극에 따른 집단 P300의 진폭값은 비 연합자극보다 높았다. P300... -
“These are my camera glasses” : Wearable Digital Video Glasses for Recording and Examining Young Children’s Social Interactions
Christina Davidson;Amy MacDonald;Angela Fenton 환태평양유아교육연구학회 Asia-Pacific journal of research in early childhood education 27 Pages
환태평양유아교육연구학회 Asia-Pacific journal of research in early childhood education 2018, Vol.12 No.3 5 93-119 (27 pages)
As new technologies emerge and evolve, possibilities open up for their use in research. In this article, we consider wearable digital video glasses as a data collection tool in qualitative research involving child participants. We draw on data from a recording made during our pilot study of the ways young children’s social interactions produced learning opportunities during family shopping experiences. The recording was produced when the digital video glasses were worn by one six-year old boy...


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