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Mechanism of Pituitary Adenylate Cyclase-Activating Polypeptide- Induced Inhibition on Catecholamine Secretion Evoked by Cholinergic Stimulation and Membrane Depolarization in the Rat Adrenal Gland
Dong-YoonLim, Jeong-WonKang, Young-JoKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 12 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1999, Vol.3 No.3 12 339-350 (12 pages)
The present study was attempted to examine the effect of pituitary adenylate cyclase-activating polypeptide (PACAP) on catecholamine (CA) secretion evoked by cholinergic stimulation, membrane depolarization and calcium mobilization from the isolated perfused rat adrenal gland. The perfusion of PACAP (10 nM) into an adrenal vein for 60 min produced a great inhibition in CA secretion evoked by ACh (5.32⁓103 M), high K (5.6⁓102 M), DMPP (104 M for 2 min),... -
Thyroid Hormone-Induced Alterations of Ryanodine and Dihydropyridine Receptor Protein Expression in Rat Heart
HaeWonKim, MiyoungPark, EunHeeLee, HyoungJinCho, HeeRanLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1999, Vol.3 No.3 11 329-337 (9 pages)
Thyroid hormone-induced cellular dysfunctions may be associated with changes in the intracellular Ca2 concentration. The ryanodine receptor, a Ca2 release channel of the SR, is responsible for the rapid release of Ca2 that activates cardiac muscle contraction. In the excitation-contaction coupling cascade, activation of ryanodine receptors is initiated by the activity of sarcolemmal Ca2 channels, the dihydropyridine receptors. In hyperthyroidism left ventricular... -
Opening of ATP-sensitive K+ Channel by Pinacidil Requires Serine/ Threonine Phosphorylation in Rat Ventricular Myocytes
Yong-GeunKwakSoo-WanChae 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 11 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1999, Vol.3 No.3 7 293-303 (11 pages)
The influences of specific protein phosphatase and protein kinase inhibitors on the ATP-sensitive K (KATP) channel-opening effect of pinacidil were investigated in single rat ventricular myocytes using patch clamp technique. In cell-attached patches, pinacidil (100 μM) induced the opening of the KATP channel, which was blocked by the pretreatment with H-7 (100 μM) whereas enhanced by the pretreatment with genistein (30 μM) or tyrphostin A23 (10 μM). In inside-out patches, pinacidil... -
Increase of Intracellular Ca2+ Concentration by Vibrio Vulnificus Cytolysin in Rat Platelets; Triggering Mechanism of Platelet Cytolysis
JinBongParkSooWanChae 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1999, Vol.3 No.2 10 199-205 (7 pages)
Vibrio vulnificus cytolysin caused platelet cytolysis and increased intracellular calcium concentration ([Ca2]]i) of rat platelets in a concentration-dependent manner. In the presence of V. vulnificus cytolysin (3 HU/ml), lactate dehydrogenase (LDH) activity was increased from 1.3⁑0.4% of control to 64.3⁑3.4% in platelet suspension buffer. In Ca2-free platelet suspension buffer, however, V. vulnificus cytolysin did not induce [Ca2]i increase and LDH release.... -
Effects of Ethanol on Na+-dependent Solute Uptake in Rabbit Renal Brush-Border Membrane Vesicles
YongKeunKim, SunHeeKo, JaeSukWoo, JinSupJung, SangHoLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1999, Vol.3 No.2 9 191-198 (8 pages)
This study was undertaken to examine the effect of ethanol on Na-dependent transport systems (glucose, phosphate, and dicarboxylate) in renal brush-border membrane vesicles (BBMV). Ethanol inhibited Na- dependent uptakes of glucose, phosphate, and succinate in a dose-dependent manner, but not the uptakes of Na-independent. The H/TEA antiport was reduced by 8% ethanol. Kinetic analysis showed that ethanol caused a decrease in Vmax of three transport systems, leaving... -
Role of Phospholipase A2 in Hypoxia-Induced Renal Cell Injury
WonRakChoi, SunHeeKo, SuInCho, JaeSukWoo, JinSupJung, SangHoLee, YongKeunKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1999, Vol.3 No.1 12 93-100 (8 pages)
The present study was designed to assess the roles of PLA2 activation and arachidonic acid (AA) metabolites in hypoxia-induced renal cell injury. Hypoxia increased LDH release in a dose-dependent manner in rabbit renal cortical slices, and this increase was significant after 20-min hypoxia. The hypoxia-induced LDH release was prevented by amino acids, glycine and alanine, and extracellular acidosis (pH 6.0). Buffering intracellular Ca2 by a chelator, but not omission of Ca2 in the... -
Regulation of Magnesium Release by cAMP during Chemical Hypoxia in the Rat Heart and Isolated Ventricular Myocytes
JinShangKimAntonioScarpa 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 10 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1999, Vol.3 No.1 8 59-68 (10 pages)
Chemically induced hypoxia has been shown to induce a depletion of ATP. Since intracellular free Mg2 ([Mg2]i) appears to be tightly regulated following cellular energy depletion, we hypothesized that the increase in [Mg2]i would result in Mg2 extrusion following hormonal stimulation. To determine the relation between Mg2 efflux and cellular energy state in a hypoxic rat heart and isolated myocytes, [Mg2]i, ATP and Mg2 content were measured by... -
Modulation of ATP-Induced Activation of the Muscarinic K+ Channel Activity by Protein Kinase C
Yang-MiKim, Hong-KiPark, Jae-HeeHan, Choon-OkPark, Seong-GeunHong 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 11 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.6 11 743-753 (11 pages)
The atrial acetylcholine-activated K (KACh) channel is gated by the pertussis toxin-sensitive inhibitory G (GK) protein. Earlier studies revealed that ATP alone can activate the KACh channel via transphosphorylation mediated by nucleoside-diphosphate kinase (NDPK) in atrial cells of rabbit and guinea pig. This channel can be activated by various agonists and also modulated its function by phosphorylation. ATP-induced KACh channel activation (AIKA) was maintained in the presence of the... -
Increase of L-type Calcium Current by cGMP-dependent Protein Kinase Regulates in Rabbit Ventricular Myocytes
JinHan, NariKim, EuiyongKim, WonkyungHo, YungEEarm, HankyounKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 10 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.6 10 733-742 (10 pages)
in rabbit ventricular myocytes using whole-cell voltage clamp method. ICa was elicited by a depolarizing pulse to 10 mV from a holding potential of 40 mV. Extracellular 8-(4-Chlorophenylthio)-guanosine-3',5'-cyclic monophosphate (8-pCPT-cGMP), potent stimulator of cGMP-dependent protein kinase (cGMP-PK), increased basal ICa. cGMP-PK also increased basal ICa. The stimulation of basal ICa by cGMP-PK required both 8-Br-cGMP in low concentration and intracellular ATP to be present. The... -
Effects of Neonatal Footshock Stress on Glucocorticoid and 5- HT2A/2C Receptor Bindings and Exploratory Behavior
DongGooKim, SeoulLee, DongWonKang, JongSuLim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.6 4 677-685 (9 pages)
To investigate the effects of neonatal stress on behavior and neurochemistry, rats were exposed to the footshock stress on postnatal day (PND) 14 or PNDs 14 and 21. Rats were exposed to uncontrollable electric shocks delivered to the floor with a constant current (0.8 mA) for 5 sec period. Daily sessions consisted of 60 trials on a random time schedule with an average of 55 sec. The first exposure to footshocks on PND 14 decreased body weight gain for 1 day. However, the second exposure to...
