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Overexpressed Mitochondrial Thioredoxin Protects PC12 Cells from Hydrogen Peroxide and Serum-deprivation
Yun-SongLee, Seung-AYu 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 5 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2003, Vol.7 No.1 7 33-37 (5 pages)
conserved active site, Cys-X-X-Cys. Whereas biological and physiological properties of Trx1 are well characterized, significance of mitochondrial thioredoxin (Trx2) is not well known. Therefore, we addressed physiological role of Trx2 in PC12 cells under oxidative stress. In PC12 cells, transiently overexpressed Trx2 significantly reduced cell death induced by hydrogen peroxide, whereas mutant Trx2, having serine residues instead of two cysteine residues at the active site did not. In addition,... -
Interactions between Estradiol-17β-BSA and Calcitropic Hormones in Ca2+ Uptake in Renal Proximal Tubule Cells
HoJaeHan, YeunHeeLee, EunJuSeo 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2002, Vol.6 No.5 5 261-268 (8 pages)
on the E2-BSA-induced stimulation. E2-BSA significantly increased cAMP generation and PKC activity. The stimulatory effect of cotreatment of E2-BSA and PTH or vitamin D was blocked by SQ22536 (an adenylate cyclase inhibitor) and staurosporine (a PKC inhibitor), but the effect of cotreatment of E2-BSA and calcitonin was not blocked. Furthermore, 8-Br-cAMP and TPA (an artificial PKC promoter) increased Ca2 uptake by 25.51% and 16.47%, respectively, compared with the control. In conclusion,... -
Lipid Peroxidation, NF-κB Activation and Cytokine Production in Neutrophil-Stimulated Pancreatic Acinar Cells
HyeyoungKim, JeongYeonSeo, SeHaengCho, KyungHwanKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1999, Vol.3 No.5 9 521-528 (8 pages)
in the absence or presence of NAC (10 mM) or SOD (300 U/ml). As a result, the productions of H2O2, LPO and TNF-α were increased with the ratio of PMA-primed neutrophils to acinar cells while the productions of LPO, IL-1β, IL-6 and TNF-α were increased with time. PMA-primed neutrophils resulted in the activation of NF-κB. Both NAC and SOD inhibited neutrophil-induced alterations in acinar cells. In conclusion, ROS, generated by neutrophils, activates NF-κB, resulting in upregulation of... -
Acute Pulmonary Responses in Vivo to Silica Complexed with H+, Zn2+, or Fe3+
JiheeLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1999, Vol.3 No.2 8 183-189 (7 pages)
in red blood cells and total leukocytes among any of the cation complexed silica groups. The levels of LDH and total protein in the lavage fluid were significantly increased by 3- to 4-fold. However, compared among these silica groups, Fe3 complexation did not significantly change the LDH activity and total protein. NO production in cultured bronchoalveolar lavage cells was elevated by 2-fold, following IT any of the silica treatments compared with the saline group. Furthermore, the... -
Inducible Nitric Oxide Synthase mRNA Expression and Nitric Oxide Production in Silica-Induced Acute Inflammatory Lung Injury
JiheeLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.2 12 233-239 (7 pages)
to silica, differential counts of broncho- alveolar lavage cells and lactate dehydrogenase(LDH) activity as well as total protein in the broncho-alveolar lavage fluid were determined. Broncho-alveolar lavage cells were also assayed for iNOS mRNA and the productions of nitrite and nitrate measured in the cells cultured. Differential analysis of broncho-alveolar lavage cells showed that the number of alveolar macrophages slightly decreased following silica treatment; however, red blood cells,... -
Changes in Cytosolic Ca2+ but not in cGMP Contents May be more Important to Nitric Oxide-Mediated Relaxation in Depolarized Vascular Smooth Muscle
HyunSeokLeeKiChurlChang 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.1 8 63-68 (6 pages)
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백서 뇌 별아교세포에서 LipopolysaccharideI에 의한 Nitric Oxide 생성기전
소광, 박래길, 이상렬, 박민철 대한신경정신의학회 신경정신의학 13 Pages
대한신경정신의학회 신경정신의학 1999, 제 38권 제 4호 20 881-893 (13 pages)
연구목적： Nitric oxide(NO)는 뇌졸중 및 여러 가지 신경변성 질환의 병리생리에 중요한 역할을 한다. 본 연구는 백서 뇌로부터 분리한 별아교세포에서 세균내독소 lipopolysaccharide(LPS)에 의한 NO 생성과정이 MAP kinase cascades와 전사 활성인자 NF-kB에 의해 중재됨을 밝히고자 하는 것이다. 방 법： NO 생성은 Greese’s reagents와 Western blot analysis를 사용하여 측정하였고 Erk, JNK1, p38은 in vitro immunecomplex kinase assay로 측정했다. NF-kB의 전사적 활성은 electrophoretic mobility shift assay로 결정하였다.... -
아세트아졸아마이드 부하 뇌 단일광자방출 전산화단층촬영으로 평가한 알쯔하이머형 치매에서의 뇌혈관 반응성
이동우, 이재성, 이동수, 정준기, 이명철, 조경형, 정희연, 조맹제 대한신경정신의학회 신경정신의학 10 Pages
대한신경정신의학회 신경정신의학 2002, 제 41권 제 6호 6 1010-1019 (10 pages)
연구목적： 본 연구의 목적은 Acetazolamide 부하 뇌 단일광자 방출 전산화 단층촬영(Brain Single Photon Emission Computed Tomography：Brain SPECT)을 알쯔하이머형 치매 환자군과 정상대조군을 대 상으로 시행한 후 여기에서 얻어진 영상을 3차원적인 관심용적 접근법으로 분석함으로써 알쯔하이머 형 치매에서의 뇌혈관반응성의 이상 유무를 규명하기 위한 것이다. 방 법： 60세 이상의 알쯔하이머형 치매 환자군 18명과, 60세 이상의 정상대조군 10명을 대상으로 하였다. 알쯔하이머형 치매 환자군은 정신과적 면담을 통해... -
레세르핀이 副賢과 腦幹에서 티로신 수산화효소와 페닐에타놀아민 엔-메틸전이효소의 組織特異發顯에 미치는 影響에 관한 研究
김무진, 우종인, 서유헌, 이정균 대한신경정신의학회 신경정신의학 15 Pages
대한신경정신의학회 신경정신의학 1994, 제 33권 제 6호 22 1432-1446 (15 pages)
