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전체 선택해제
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본문검색 결과만 보기
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Graphene Based Electrochemical DNA Biosensor for Detection of False Smut of Rice (Ustilaginoidea virens)
Kritika Rana, Jagjiwan Mittal, Jagriti Narang, Annu Mishra, Ramesh Namdeo Pudake 한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 3호 8 291-298 (8 pages)
analysis tools namely, cyclic voltammetry, and linear sweep voltammetry. To confirm the selectivity of the device, probe hybridization with non-complementary ssDNA target has been studied. In our study, the developed sensor was able to detect up to 10 fM of target ssDNA. The paper electrodes were employed to pro- duce an effective and cost-effective platform for the immobilization of the DNA and can be extended to design low-cost biosensors for the detection of the other plant pathogens. -
Identification of Heterodera glycines (Tylenchida; Heteroderidae) Using qPCR
Hyoung-Rai Ko, Heonil Kang, Eun-Hyoung Park, Eun-Hwa Kim, Jae-Kook Lee 한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 6호 11 654-661 (8 pages)
of DNA extracted from a single cyst was obtained for the sensitivity test. The result showed that the standard curve of the test had a highly significant linearity between DNA concentration and Ct value (R2 = 0.996, slope = −3.49) and that the detection limit concentration of DNA of the primer set was 10 pg of DNA per reaction. Our findings suggested that H. glycines could be distinguished from H. sojae and other Heterodera species when a qPCR assay is used with a specific primer set. -
Genomics-based Sensitive and Specific Novel Primers for Simultaneous Detection of Burkholderia glumae and Burkholderia gladioli in Rice Seeds
Chae-yeong Lee, Hyun-Hee Lee, Mohamed Mannaa, Nam-gyu Kim, Jung-wook Park, Ju-yun Kim, Young-Su Seo 한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2018, 34권 6호 4 490-498 (9 pages)
size. Both primer pairs displayed a high degree of sensitivity for detection of B. glumae and B. gladioli separately in monoplex PCR or simultaneously in duplex PCR from both extracted gDNA and directly preheated bacterial cell suspensions. Limit of detection was as low as 0.1 ng of gDNA of both species and 3.86 × 102 cells for B. glumae and 5.85 × 102 cells for B. gladioli. On inoculated rice seeds, the designed primers could separately or simultaneously detect B. glumae and B. gladioli with... -
Simple Detection of Cochliobolus Fungal Pathogens in Maize
In Jeong Kang, Hyeong Kwon Shim, Jae Hwan Roh, Sunggi Heu, Dong Bum Shin 한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2018, 34권 4호 7 327-334 (8 pages)
be used for duplex PCR analysis. This duplex PCR-based method was found to be effective for diagnosing simultaneous infections from the two Cochliobolus species that display similar morphological and mycological characteristics. With this method, it is possible to prevent infections in maize by detecting infected seeds or maize and discarding them. Besides saving time and effort, early diagnosis can help to prevent infections, establish comprehensive management systems, and secure healthy seeds. -
Validation and Application of a Real-time PCR Protocol for the Specific Detection and Quantification of Clavibacter michiganensis subsp. sepedonicus in Potato
한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2015, 31권 2호 4 123-131 (9 pages)
Clavibacter michiganensis subsp. sepedonicus (Cms) multiplies very rapidly, passing through the vascular strands and into the stems and petioles of a diseased potato. Therefore, the rapid and specific detection of this pathogen is highly important for the effective control of the pathogen. Although several PCR assays have been developed for detection, they cannot afford specific detection of Cms. Therefore, in this study, a computational genome analysis was performed to compare the sequenced... -
Begomoviruses and Their Emerging Threats in South Korea: A Review
한국식물병리학회 The Plant Pathology Journal 14 Pages
한국식물병리학회 The Plant Pathology Journal 2012, 28권 2호 1 123-136 (14 pages)
Diseases caused by begomoviruses (family Geminiviridae, genus Begomovirus) constitute a serious constraint to tropical and sub-tropical agro-ecosystems worldwide. In recent years, they have also introduced in temperate regions of the world where they have great impact and are posing a serious threat to a variety of greenhouse crops. Begomoviral diseases can in extreme cases reduce yields to zero leading to catastrophic losses in agriculture. They are still evolving and pose a serious threat to... -
Examining Teacher Collegiality in Context
함승환, Seung-Hwan Ham 한국교육행정학회 교육행정학연구 23 Pages
한국교육행정학회 교육행정학연구 2011, 제 29권 제 2호 9 135-157 (23 pages)
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School Effects
양정호 한국교육사회학회 한국교육사회학회 학술대회자료집 47 Pages
한국교육사회학회 한국교육사회학회 학술대회자료집 2002 춘계학술대회 4 93-139 (47 pages)
This study investigates the ways in which quantitative social scientists have attempted to examine school effects on student achievement. Since the 1966 publication of the Coleman Report, school effects research broadly categorized as input-output studies -
A Choice of Nightmares: The Fisher King and the Horror in Heart of Darkness and Apocalypse Now
Youngkul Kim 이화여자대학교 교과교육연구소 Eastwest Education 46 Pages
이화여자대학교 교과교육연구소 Eastwest Education 2006, Vol.23 2 13-58 (46 pages)
This study investigates Joseph Conrad's Heart of Darkness and its film adaptation, Francis Ford Coppola’s Apocalypse Now, by examining the character of the Fisher King, a common figure at the beginning of human society who was later incorporated into romance literature, especially in the quest for the Holy Grail in the Arthurian legend. A superior warrior of godlike proportions, he leads his people to health and prosperity; as Fisher King, however, he becomes sick, and that sickness is...
