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Specific and Sensitive Primers Developed by Comparative Genomics to Detect Bacterial Pathogens in Grains
한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2018, 34권 2호 3 104-112 (9 pages)
blight of maize, Stewart’s wilt of maize and spike blight of wheat and barley, respectively. The bacterial diseases are not globally distributed and not present in Korea. This study adopted comparative genomics approach and aimed to develop specific primer pairs to detect these three bacterial pathogens. Genome comparison among target pathogens and their closely related bacterial species generated 15–20 candidate primer pairs per bacterial pathogen. The primer pairs were assessed... -
Development of a Species-specific PCR Assay for Three Xanthomonas Species, Causing Bulb and Flower Diseases, Based on Their Genome Sequences
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2015, 31권 3호 2 212-218 (7 pages)
a species-specific PCR assay for rapid and accurate detection of three Xanthomonas species, X. axonopodis pv. poinsettiicola (XAP), X. hyacinthi (XH) and X. campestris pv. zantedeschiae (XCZ), based on their draft genome sequences. XAP, XH and XCZ genomes consist of single chromosomes that contain 5,221, 4,395 and 7,986 protein coding genes, respectively. Species-specific primers were designed from variable regions of the draft genome sequence data and assessed by a PCR-based detection method.... -
Validation and Application of a Real-time PCR Protocol for the Specific Detection and Quantification of Clavibacter michiganensis subsp. sepedonicus in Potato
한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2015, 31권 2호 4 123-131 (9 pages)
of a subspecies-specific PCR primer set (Cms89F/R). The specificity of the primer set based on the putative phage-related protein was evaluated using genomic DNA from seven isolates of Cms and 27 other reference strains. The Cms89F/R primer set was more specific and sensitive than the existing assays in detecting Cms in in vitro using Cms cells and its genomic DNA. This assay was also able to detect at least 1.47×10 copies/μl of cloned-amplified target DNA, 5 fg of DNA using genomic DNA or 10... -
Research Articles : Soybean mosaic virus Infection and Helper Component-protease Enhance Accumulation of Bean pod mottle virus-Specific siRNAs
한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 4호 3 315-323 (9 pages)
Soybean plants infected with Bean pod mottle virus (BPMV) develop acute symptoms that usually decrease in severity over time. In other plant-virus interactions, this type of symptom recovery has been associated with degradation of viral RNAs by RNA silencing, which is accompanied by the accumulation of virus-derived small interfering RNAs (siRNAs). In this study, changes in the accumulation of BPMV siRNAs were investigated in soybean plants infected with BPMV alone, or infected with both BPMV... -
Syntactic Difficulties in Children with Specific Language Impairment (SLI); why do they have difficulties in complex sentences and what are the intervention options?
Dong Sun Yim 이화여자대학교 교과교육연구소 Eastwest Education 17 Pages
이화여자대학교 교과교육연구소 Eastwest Education 2013, Vol.30 1 1-17 (17 pages)
The purpose of this tutorial is to provide speech-language pathologists(SLPs) with updated general information regarding language intervention techniques for children with syntactic language impairment. This clinical resource was compiled to support SLPs who need to understand the functions and effects of diverse interventions available for children with syntactic language impairment. Intervention studies have demonstrated that treatment of these deficits is indeed effective, and to date, the... -
Production of a highly group-specific monoclonal antibody against zearalenone and its application in an enzyme-linked immunosorbent assay
Sang-Ho Cha, Sung-Hee Kim, Karyn Bischoff, Hyun-Jeong Kim, Seong-Wan Son, Hwan-Goo Kang* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 2 119-125 (7 pages)
measured with standard solutions was 10 ppb. The intra-plate and inter-well variation of both ELISAs were less than 10%. The IC50 values for a-zearalenol, b-zearalenol, a-zearalanol, and b-zearalanol compared to ZEN were 108.1, 119.3, 114.1, and 130.3% for the ZEN-coated ELISA. These values were 100.7, 120.7, 121.6, and 151.6% for the anti-ZEN antibody-coated ELISA. According to the anti-ZEN antibody- coated ELISA, the average recovery rates of ZEN from spiked animal feed containing 150 to 600... -
Molecular differentiation of Korean Newcastle disease virus (NDV) by restriction enzyme analysis and pathotype-specific RT-PCR
HyukJoonKwon, SunHeeCho, SunJoongKim 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 9 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2006, 제 46권 제 4호 10 371-379 (9 pages)
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p38 MAPK Participates in Muscle-Specific RING Finger 1-Mediated Atrophy in Cast-Immobilized Rat Gastrocnemius Muscle
JunghwanKim, Kyung-JongWon, HwanMyungLee, Byong-YongHwang, Young-MinBae, WhanSooChoi, HyukSong, KiWonLim, Chang-KwonLee, BokyungKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2009, Vol.13 No.6 13 491-496 (6 pages)
of muscle-specific RING finger 1 (MuRF1) during atrophy of the rat gastrocnemius muscle. Histological analysis revealed that cast immobilization induced the atrophy of the gastrocnemius muscle, with diminution of muscle weight and cross-sectional area after 14 days. Cast immobilization significantly elevated the expression of MuRF1 and the phosphorylation of p38 MAPK. The starvation of L6 rat skeletal myoblasts under serum-free conditions induced the phosphorylation of p38 MAPK and the... -
Comparison of allele-specific PCR, created restriction-site PCR, and PCR with primer-introduced restriction analysis methods used for screening complex vertebral malformation carriers in Holstein cattle
Kozet Avanus, Ahmet Altınel 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 4호 9 465-470 (6 pages)
of allele-specific polymerase chain reaction (AS-PCR), created restriction-site PCR (CRS-PCR), and PCR with primer-introduced restriction analysis (PCR-PIRA), three methods used in identification of CVM carriers in a Holstein cattle population. In order to screen for the G>T mutation in the solute carrier family 35 member A3 (SLC35A3) gene, DNA sequencing as the gold standard method was used. The prevalence of carriers and the mutant allele frequency were 3.2% and 0.016, respectively, among...


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