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Effects of Ethanol on Na+-dependent Solute Uptake in Rabbit Renal Brush-Border Membrane Vesicles
YongKeunKim, SunHeeKo, JaeSukWoo, JinSupJung, SangHoLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1999, Vol.3 No.2 9 191-198 (8 pages)
Kinetic analysis showed that ethanol caused a decrease in Vmax of three transport systems, leaving Km values unchanged. Ethanol decreased phlorizin binding, which was closely correlated with the decrease in Vmax of Na-glucose uptake. These results indicate that ethanol inhibits Na-dependent uptakes of glucose, phosphate, and dicaboxylate and that the reduction in Vmax of Na-glucose uptake is caused by a decrease in the number of active carrier proteins in the membrane. -
Acute Pulmonary Responses in Vivo to Silica Complexed with H+, Zn2+, or Fe3+
JiheeLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1999, Vol.3 No.2 8 183-189 (7 pages)
among any of the cation complexed silica groups. The levels of LDH and total protein in the lavage fluid were significantly increased by 3- to 4-fold. However, compared among these silica groups, Fe3 complexation did not significantly change the LDH activity and total protein. NO production in cultured bronchoalveolar lavage cells was elevated by 2-fold, following IT any of the silica treatments compared with the saline group. Furthermore, the steady-state levels of iNOS mRNA in the lava... -
Calcium Modulates Excitatory Amino Acid (EAA)- and Substance P-induced Rat Dorsal Horn Cell Responses
HongKeeShin, SokHanKang, InDukChung, KeeSoonKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 11 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1999, Vol.3 No.1 5 35-45 (11 pages)
EGTA suppressed the response of WDR cell to NMDA. SP had triple effects on the spontaneous activity as well as NMDA-induced responses of WDR cells: excitation, inhibition and no change. EGTA augmented, but Ca2, Mn2 and verapamil suppressed the increase in the NMDA-induced responses and spontaneous activities of WDR cells following iontophoretical application of SP. These results suggest that in the spinal cord, sensory informations mediated by single or combined action of EAA and... -
Effect of Endothelin-1 on Proliferation and Differentiation of Rat Tracheal Epithelial Cells
ChangSooKim, SaeOckOh, JaeSukWoo, JinSupJung, YongKeunKim, SangHoLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.6 13 763-770 (8 pages)
in tracheal epithelial cells. However, the effect of endothelin-1 on the proliferation and differentiation of tracheal epithelial cells has not been clearly elucidated. This study was undertaken to investigate these actions of endothelin-1 in primary cultured cells of rat tracheal epithelia. Endothelin-1 stimulated proliferation of tracheal epithelial cells 1.5-fold when compared with that of control cells. Endothelin-1 increased mitogen-activated protein kinase (MAPK) activity. Herbimycin A, a... -
Moderate Elevation of Extracellular K+ Concentration Induces Vasorelaxation in Isolated Rat, Rabbit and Human Cerebral Arteries Role of Na Pump and Ba-Sensitive Process
DongHoKim, SungJoonKim, SangJinLee, SungJinPark, KiWhanKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 10 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.6 7 705-714 (10 pages)
and humans. Addition of K (2∼10 mM) to the control solution induced vasorelaxations. These effects were inhibited by the pretreatment with both ouabain (10 ㄍM) and Ba2 (0.1∼0.3 mM) in the rat, but only with ouabain (10 ㄍM) in the rabbit and human. These results suggest that K-induced vasorelaxation occurs via the stimulation of electrogenic Na pump in the rabbit and human MCAs, while in the rat MCAs via the activation of both Na pump and Ba-sensitive process. -
Altered Cerebral Vasomotion with Decreased CGRP Level in Pial Arteries of Spontaneously Hypertensive Rats
KwangHoLee, JaeMoonChoi, KiWhanHong 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.5 5 573-580 (8 pages)
25.5⁑3.1 and 24.6⁑3.1 fmole/mm2/60 min, respectively. The isolated basilar arteries showed similar results to those of the pial arteries in SHR. Thus, it is summarized that, in the SHR, the reduced autoregulatory vasodilator responses to stepwise hypotension and capsaicin may be, in part, ascribed to the decreased release of CGRP from the perivascular sensory nerve fibers of the pial arteries, and that altered vasomotor activity in SHR may not be related with the hypertensive tone. -
Protective Mechanism of Nitric Oxide and Mucus against Ischemia/ Reperfusion-Induced Gastric Mucosal Injury
HyeyoungKim, KwangsooNam, KyungHwanKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.4 13 511-519 (9 pages)
activities of gastric mucosa were determined. Histological observation of gastric mucosa was performed by using hematoxylin-eosin staining and scanning electron microscopy. The result showed that ischemia/reperfusion increased lipid peroxide production and decreased the contents of glutathione and mucus as well as glutathione peroxidase activities of gastric mucosa. Ischemia/reperfusion induced gastric erosion and gross epithelial disruption of gastric mucosa. Pretreatment of L-arginine, a... -
Rapid Induction of mRNA for Prostaglandin H Synthase in Ovine Meningeal Fibroblasts
MyeongJinNam, ClaraThore, DavidBusija 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.4 5 435-441 (7 pages)
after 60 min., and 355⁑32 pg/ml after 90 min (all p<0.05) (n=6). Actinomycin-D (1 mg/ml) prevented increases in mRNA, 6-keto-PGF1α, and PGF2α at 60 min. for both IL1α and PDB. We conclude that cerebral fibroblasts are avid producers of prostanoids, and that enhanced production of PGHS is responsible for augmented PGF2α and prostacyclin production in the presence of an activator of protein kinase C and for decreased PGE2 and increased prostacyclin production in the presence of IL1α. -
Effects of Exogenous ATP on Calcium Mobilization and Cell Proliferation in C6 Glioma Cell
Eun-JungLee, SeokHoCha, WoonKyuLee, Kweon-HaengLee, Sang-BokLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.4 3 419-425 (7 pages)
To clarify the effect of extracellular ATP in cultured C6 glioma cells, ATP-induced cytosolic free calcium ([Ca2]i) mobilization and cell proliferation were investigated. ATP-induced [Ca2]i increased in a dose-dependent manner (107 M∼103 M). ATP-induced [Ca2]i increases were slightly slowed in extracellular calcium-free conditions especially in sustained phase. ATP-induced [Ca2]i increment was also inhibited by the pretreatment of U73122, a... -
Inducible Nitric Oxide Synthase mRNA Expression and Nitric Oxide Production in Silica-Induced Acute Inflammatory Lung Injury
JiheeLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.2 12 233-239 (7 pages)
more highly reactive toxic species. The objectives of the present study were to evaluate acute inflammatory lung injury and to determine iNOS mRNA induction and nitric oxide production by rat broncho-alveolar lavage cells following intratracheal treatment of silica. After 4 h exposure to silica, differential counts of broncho- alveolar lavage cells and lactate dehydrogenase(LDH) activity as well as total protein in the broncho-alveolar lavage fluid were determined. Broncho-alveolar lavage cells...


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