밤나무산누에나방(Dictyoploca japonica)의 단백질을 acrylamide gel 전기영동법을 이용하여 분리한 결과는 다음과 같다. 1. 전 발생단계를 통하여 총 22개 (1-22)의 단백질 band가 분리되었다. 2. Band에 있어서 단백질 농도는 발생단계에 따라 차이를 나타내며 종령유충에서 증가하고 전용에서 감소하였다. 그리고 용기에서 다시 증가하는 경향을 보였다. 3. PAS 반응, Toluidine blue O, Sudan black B를 이용한 조직화학적 반응결과 종령유충에서 몇 개의 glycoprotein, acidic mucopolysaccharide, lipoprotein의 band가 확인되었다. 4. non-specific esterase와 acid 및 alkaline phosphatase의 band 역시 검출되었다.
The whole body proteins of camphor silk moth, Dctyoploca japonica of different developmental stages have been studied by acrylamide gel electrophoresis. A total of 22 protein bands have been identified throughout the stages. Each developmental stage exhibited both qualitative and quantitive differences in their protein patterns and, in general, the concentration of protein bands were increased at the mature larvae, and decreased at the prepuae and at the middle pupal stage they inclined to be increased. Histochemical staining of the acrylamide gels by the PAS method, Toluidine blue O, and Sudan black B demonstrated that the carbohydrate, acidic mucop-loysaccharide, and lipid were associated with certain body proteins. In addition, non-specific esterases, acid and alkaline phosphatases also were identified.
