Methadone 대사물질의 GCMS 검출 및 확인을 위하여 중수소로 치환된 methadone 및 그 대사물질을 이용하였다. methadone을 투여한 쥐에서 얻은 bile의 conjugate fraction에서 m/z 72를 함유한 물질을 검출하였고 GCMS data를 근거로 하여 normethadone nitrone (N-methylene-1-methyl-3, 3-diphenyl-4-oxo-hexanamine-oxide)으로 확인하였다. 이 물질은 sample 처리과정에서 생긴 N-hydroxynormethadone의 분해 산물로 추청된다. N-hydroxynormethadone의 대사에 의한 생성 가능성 및 그 중요성을 기술하였다.
The deuterium labeled methadone and metabolites were used for GCMS (Gas Chromatography Mass Spectrometry) detection and identification of methadone metabolites. The conjugated fraction from methadone dosed rat bile was found to contain a new m/z 72 containing compound. On the basis of GCMS data the new compound was assigned as normethadone nitrone (N-methylene-1-methyl-3, 3-diphenyl-4-oxo-hexanamineoxide). The nitrone appears to result from decomposition of N-hydroxynormethadone during sample preparation procedures. Possibilities for the metabolic formation of N-hydroxynormethadone and its physiological significance were discussed.
