To find out the effect of transplanting time on the autonomic characters, yield of cured leaves and content of polyphenols in burley tobacco, three transplanting time were evaluated. The results obtained are as follows. Low temperature which was caused damage earliest stage of tobacco in early transplanting could escape by vinyl mulching and other treatment. 2. The major polyphenols in leaves were chlorogenic acid and rutin, small amount of scopolin and quercetin were also detected. 3. Chlorogenic acid decreased during curing. 4. Polyphenols content was not influenced by transplanting time.