This study was carried out to find out the variations in Burley 21 variety of Surely tobacco. Jeonjul and nine other selected lines of Burley 21 were evaluated. Plant height and several other characteristics were significantly different among tested lines. Especially, Jangsu-1, the earliest flowered line, flowered 5 to 7 days earlier than other lines and had the smallest length width ratio of the largest leaf. Narrow-leaved type, whose length/width ratio of the largest leaf was above 2, 16, was significantly different in number of leaves and weight per unit leaf area from broad-leaved type whose length/width ratio of the largest leaf being below 1.97. Among tested lines, Jeonju (2) - 2 was the best in major agronomic characteristics, yield and physical properties.