꼬리명주나비 (Cericinus montela)의 발생 단계에 따라 혈림프의 단백질 pattern과 가수분해 효소의 zymogram을 1차원 및 2차원전기영동법을 이용하여 연구한 결과는 다음과 같다. 1. 1차원 및 2차원전기영동법에 의하여 분리된 단백질의 band와 spot들은 분자량 669~13kd에 해당하였다. 2. 전 발생 단계를 통하여 총 41개의 단백질 band와 130~357개의 단백질 spot가 확인되었며, 발생 단계에 따른 단백질 band와 spot 수의 변화는 서로 유사하였다. 3. 분리된 단백질 band와 spot 중에서 14개의 band와 84개의 spot는 전 단계에서 한결같이 확인되었으며, 특히 유충조직의 histolysis와 성충조직의 histogenesis에 관련되는 것으로 여겨지는sport group들이 특정 단계에서 출현되거나 소실되었다. 4. 종령유충에서 용 1일로의 발생 단계를 통하여 7개의 esterase, 6개의 acid phosphatase, 7개의 alkaline phosphatase 그리고 9개의 leucinaminopetidase의 band가 조직화학적 염색법에 의하여 확인되었는데, 그들의 pattern상은 용화와 관련하여 변화하였다.
The protein patterns and hydrolase zymograms of haemolymph in Cericinus montela during development were investigated by means of one- and two-dimensional electrophoresis. The results obtained are as follows ; 1. The protein bands and spots separated by using of one- and two-dimensional electrophoresis have molecular weight between 669 and 13 kd. 2. A total of 41 protein bands after one-dimensional electrophoresis and 130 to 357 protein spots after two-dimensional electrophoresis have been identified throughtout the development, and the changes in the number of protein bands and spots were similar together. 3. Among the above protein bands and spots, 14 bands and 84 spots appeared constantly in all the stages. In particular, the other groups of protein spots which are considered to be related to the histolysis of larval tissues and histogenesis of adult tissues were appeared or disappeared in specific stages. 4. By means of histochemical staining methods, 7 esterase, 6 acid phosphatase, 7 alkaline phosphatase and 9 leucinaminopeptidase bands were found during larval-pupal development and their patterns were changed in relation to pupation.
