In this study, vibrational behavior of uniform pipe carrying a moving medium is studied by using a transfer matrix and the displacement function derived from the conventional beam theory. In various boundary conditions, flow velocity and mechanical property change of the variation of natural frequency are investigated. The Coriolis term in the original differential equation of motion has been ignored in the investigation. This method is used to study the variation of natural frequency with flow velocity for clamped-clamped, cantilevered, clamped-pinned, pinned-pinned, free-free straight pipe element. It is shown that clamped-clamped, free-free pipe have the highest natural frequency and critical velocity values while cantilevered pipe have the smallest natural frequency for the same mechanical properties. From the vibration effects of mechanical property variation, it is shown that bending stiffness and pipe length variation has large influence on natural frequency and critical velocity. Since the order of transfer matrix is not changed with boundary conditions of pipe element, this method proposed can be easily applied to personal-computer for vibration analysis of pipe element. Furthermore, this method can be extended to three-dimensional system by using a coordinate transformation for the analysis of piping systems.