The present study was to carried out to investigate the anti-stress effects of Samhwasan in immobilized rats. Serum levels of catecholamine and aldosterone were measured in immobillzed rats orally receiving extracts of Samhwasan, respectively, for 1 day 7 days. And in order to investigate the analgesic effects of Samhwasan in acetic-acid injected mice, the writhing syndrome frequency was measured. The results obtained were summarized as follow ; 1. There were decreases of norepinephrine in rats receiving Samhwasan for 1 and 7 days respectively. 2. There were decreases of epinephrine in rats receiving Samhwasan for 1 day and 7 days respectively, and significant decreases were recognized in rats receiving Samhasan for 7 days. 3. There were significant decreases of aldosterone in rats receiving Samhwasan for 1 day and 7 days respectively. 4. There were significnat decreases of writhing syndrome frequency in mice receiving Samhwasan. These results appeared to suggest that Samhwasan produce the anti-stress effects and effects on pain relief.