Characteristics of flow and heat transfer have been studied numerically in a square duct with a periodic pressure gradient. The flow in a duct was assumed to be fully developed and constant heat flux was imposed at the surfaces of a square duct. The distributions of axial velocity and time-space averaged temperature are investigated with angular velocity and amplitude ratio at a given Reynolds number 1000. When the periodic pressure gradient was imposed axially in a duct, the reverse flow may be occurred near the duct wall. The magnitude of this reverse flow increases as the amplitude ratio increases or as the angular frequency decreases. In the ranges of the amplitude ratio and the angular velocity in present investigation, the ratio of the periodic time space averaged temperature to the nonperiodic space averaged temperature has been found to be greater than one. This means that the cooling effect at the duct walls deteriorates with a periodic situation compared with nonperiodic one.