배추흰나비 용기 특이 큐티클단백질의 분포 및 합성부위를 조사하기 위해 전기영동법, 면역 확산, 조직 배양 및 Autoradiography 법으로 조사하였다. 용기 특이 큐티클단백질은 37Kd, 28Kd, 27Kd의 단백질로 확인되었으며 이중 27Kd 용기 특이 큐티클단백질은 지방체, 표피에 분포하고 있으며 지방체에서는 종령말기부터 합성되며, 전용기부터 방출되어 표피에 저장된 후 용화후부터 용기 큐티클에 축적되고 있다. 또한 37Kd와 28Kd 용기 특이 큐티클단백질은 주로 표피에 분포하고 있어 지방체에서 보다는 표피로부터 기원하는 것 같다.
The distribution and synthesis of pupal specific cuticle proteins of Pieris rapae was investigated by the method of SDS-PAGE, immuno-diffusion test, western-blot, tissue culture and autoradiography. Pupal specific cuticle proteins were identified only in pupal stage and have molecular weights of 37 Kd, 28 Kd and 27 Kd. Among these proteins 27 Kd pupal specific cuticle protein was distributed in fat body epidermis. This pupal specific cuticle protein was synthesized mainly in fat body from last larval stage and was released from just after pupal stage. But this protein was deposited in epidermis just after released from fat body and from just after ecdysis was began to incorporate into cuticle. But 37 Kd and 28 Kd pupal specific cuticle proteins were distributed mainly in epidermis and have the same electrophoretic mobilities with epidermal proteins, suggesting that these proteins are originated from epidermis rather than fat body.
