The purpose of this study was to determine the fluroide release levels of new fluoride-containing liner/base cements and the fluoride uptake by dentin surfaces. Ten specimens of each brand (Fuji ionomer Type III, Fuji Lining LC, Timeline, Vitrebond and XR ionomer) were made, polymerized and placed in fluoride-free distilled water at $37^{circ}C$, 100% relative humidity for 24 hours. The extracting solution of specimen was exchanged and fluoride release was measured daily for the 30 days. For fluoride uptake study, twenty-five extracted human lower molars were sectioned longitudinally in the mesiodistal direction with a diamond disc. Five teeth were filled with each material and then stored at $37^{circ}C$, 100% humidity for 4 weeks. Fluoride uptake by dentin from the test materials was evaluated using electron probe micro X-ray analyzer. The following results were obtained : 1. The amounts of fluoride release showed no significant difference between Fuji ionomer Type III and Fuji Lining LC, but showed significant difference between other groups. XR ionomer released significantly greater fluoride than any other group(P<.001). 2. All the materials have a burst effect which more fluoride released in then first 3 day and showed significant decrease over the test period (P<0.001). 3. XR ionomer group showed fluoride penetration to approximately $50{mu}m$ deep in dentin. But other material groups showed very little fluoride uptake by dentin.