배추흰나비 (Pieris rapae)의 5령 말기 유충에서 분리된 Acph$^2$에 대하여 생화학적 특성을 조사하였으며 면역학적 방법을 사용하여 발생단계에 따른 Acph$^2$의 양적변화와 다른 기관내 분포상황을 확인하였다. Acph$^2$의 최적 pH와 온도는 각각 pH 4.5와 55$^{circ}C$로 나타났으며 $K_{m}$ 값은 2$ imes$$10^{-4}$ M, 분자량은 118kD 그리고 pI값은 pH 5.8로 측정되었다. 면역학적 연구에 의한 효소활성 변화는 5령 말기 유충에서 최대로 나타났으며 혈림프, 지방체, 중장 그리고 타액선에서 Acph$^2$가 존재함을 확인하였다.
The biochemical properties of Acph$^2$ which was previously purified from late 5th instar larval whole body of Pieris rapae were investigated, and the quantitative change of Acph$^2$ during the development and the distribution of Acph$^2$ in other organ were determined using immunological method. Optimal pH and temperature of acid phosphatase were found to be pH 4.5 and 55$^{circ}C$, respectively. Km value was 2$ imes$10$^{-4}$ M, molecular weight was l18kD and pI value of the enzyme was 5.8. Enzyme activity during the development by immunological study showed the highest activity at late 5th instar larvae. Acph$^2$ was present in the haemolymph, fat body, midgut and salivary grand.
